Research Article
A Submersible Holographic Microscope for 4-D In-Situ Studies of Micro-Organisms in the Ocean with Intensity and Quantitative Phase Imaging
Lina M. Rotermund1, John Samson2 and Hans Jürgen Kreuzer3*
1Department of Physics & Atmospheric Science, Dalhousie University, Halifax, NS B3H 4R2, Canada
24Deep Inwater Imaging, 6589 Chebucto Rd, Halifax, NS B3L 1L9, Canada
3Department of Physics & Atmospheric Science, Dalhousie University, Halifax, NS B3H 4R2, Canada
- *Corresponding Author:
- Hans Jürgen Kreuzer
Department of Physics & Atmospheric Science
Dalhousie University, Halifax
NS B3H 4R2, Canada
Tel: +19024946594
E-mail: h.j.kreuzer@dal.ca
Received date: January 03, 2016 Accepted date: January 21, 2016 Published date: January 27, 2016
Citation: Rotermund LM, Samson J, Kreuzer HJ (2016) A Submersible Holographic Microscope for 4-D In-Situ Studies of Micro-Organisms in the Ocean with Intensity and Quantitative Phase Imaging. J Marine Sci Res Dev 6:181. doi:10.4172/2155-9910.1000181
Copyright: © 2016 Rotermund LM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Digital holographic reconstructions yield the complex wave amplitude throughout a 3-D volume from which intensity and quantitative phase images can be obtained. A submersible digital holographic microscope with a point source has been used to demonstrate the feasibility of continuous monitoring of micro-organisms such as algae at any depth in oceans, lakes and rivers. The local refractive index can be obtained throughout the organisms. A tracking algorithm is used to study their mobility.
