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Abstract

NF-κB is a key transcriptional regulator of the inflammatory response and plays an important regulatory role in the development of chronic liver diseases. We investigated the protective effect of glycyrrhizin, omega-3 and their combination on liver fibrosis in rats through their effect on NF-κB by ELISA method and histological analysis of the extent of liver fibrosis and necroinflammatory score. Materials & methods: 50 Male Wistar rats randomized into 5 groups: Normal control group, TAA group, GL group (received Glycyrrhizin 25 mg/kg by gastric gavage daily started with TAA),Ï?-3 group(received Ï?-3 150 mg/kg by gastric gavage daily started with TAA), (GL+Ï?-3) group(received Glycyrrhizin 25 mg/kg by gastric gavage daily and Ï?-3 150 mg/kg by gastric gavage daily started with TAA), all groups except normal control group received TAA 200mg/kg i.p twice weekly for 8 weeks. Results: TAA caused liver fibrosis characterized by highly significant increase in serum AST activity, total bilirubin level, the extent of liver fibrosis and the necroinflammatory score, as well as highly significant decrease in serum albumin, total proteins levels, the fibrotic area and necroinflammatory score (P<0.005). While the GL, Ï?-3& (GL+Ï?-3) protected the liver from TAA hepatotoxic effects as they significantly prevented the increase in serum AST activity and total bilirubin level, also they significantly prevented the decrease in serum albumin and total bilirubin levels.. We tried to find the mechanism by which the glycyrrhizin and omega-3 protected the liver, TAA caused markedly high increase in the MDA level (P<0.005), while GL, Ï?-3& (GL+Ï?-3) markedly prevented this increase (P<0.005). We also found that the TAA have been markedly increased the nuclear factor kappa B concentration in the liver tissue (P<0.005), while GL, Ï?-3& (GL+Ï?-3) highly significantly decreased NF-κB concentration (P<0.005). Conclusion: these results suggested that NF-κB and oxidative stress have an important regulatory role in development of liver fibrosis. As well as, glycyrrhizin and omega 3 are potent anti-inflammatory, anti oxidative stress and anti fibrotic drugs through inhibition of oxidative stress and NF-κB pathway.

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