Our Group organises 3000+ Global Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ 黑料网 Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Photosynthesis, the process through which plants convert solar energy to chemical energy by harvesting sunlight and transfer
the excited energy from photosystems (PS) PSII and PSI, which are membrane protein complexes that helps in primary
photochemical reactions. In general abiotic stress affects the plant photosynthesis particularly salinity stress. There are number
of studies on salt effects on photosynthesis of prokaryotic and eukaryotic species which were short term treatment of different
concentrations of salt. Here we have grown the green algae C.reinhardtii cells directly in the medium containing NaCl with
different concentrations (50 mM, 100mM, and 150mM) which is more close to the natural condition. The impact of high salt
concentrations on the structural organization of photosynthetic apparatus in C.reinhardtii was studied. Confocal microscopy data
have revealed the morphology of the cells indicating that salt stress had delayed the cell division. Electron transport processes were
investigated using OJIP curves which reveals that both donor and acceptor side of PSII were impaired at moderate concentrations
of NaCl (100,150 mM).The chlorophyll a emission peak in low temperature fluorescence measurements (77K) was reduced in
increasing salt stress grown cells indicating that structural changes at the level of PSII and PSI had occurred. Further, circular
dichroism (CD) spectrum in the visible region associated with the strongly coupled chlorophyll dimers showed reduction in
intensity, indicating that pigment?pigment interactions were altered. Thylakoid protein complexes have been characterized using
BN PAGE where many of the protein complexes were down regulated in high salt conditions indicating that protein-protein
and pigment-protein interactions have changed. Further, western blot analysis of thylakoids with PSII core and light harvesting
complex antibodies have shown that antenna proteins CP47, 43 along with Lhc components Lhcb1, Lhcb4 were reduced which
indicates that both core and Lhc antenna are equally sensitive to the above optimum salt concentrations. This damage may be due
to reactive oxygen species generated in chloroplast.
Biography
Satyabala Neelam completed her M.Tech in Biotechnology in the year 2009 from University of Hyderabad, Hyderabad, Andhra Pradesh. She
is pursuing her Ph.D in Department of Biochemistry, under Dr. S. Rajagopal, Reader, Department of Plant sciences, University of Hyderabad,
Hyderabad India.
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, 黑料网 Journals
