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Studies on Effect of Chemical Preservatives on Keeping Quality of Kesar Mango Pulp

Research Article ºÚÁÏÍø
Sakhale BK1*, Pawar VN2 and Ranveer RC3
1Department of Chemical Technology, Food Technology Division, Dr.Babasaheb Ambedkar Marathwada University, Aurangabad-431004
2Faculty of Food Technology, M.A.U, Parbhani
3Department of Food Science and Technology, Shivaji University, Kolhapur, M.S., India
*Corresponding authors: Sakhale BK
Food Technology Division
Department of Chemical Technology
Dr. Babasaheb Ambedkar Marathwada University
Aurangabad-431004
Email: bksakhale@gmail.com
 
Received March 12, 2012; Published July 25, 2012
 
Citation: Sakhale BK, Pawar VN, Ranveer RC (2012) Studies on Effect of Chemical Preservatives on Keeping Quality of Kesar Mango Pulp. 1: 184. doi:10.4172/scientificreports.184
 
Copyright: © 2012 Sakhale BK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 
Abstract
 
The attempts are made in the present investigation to know the effect of various chemical preservatives on shelf life of Kesar mango pulp. The uniformly ripened Kesar mango pulp was pasteurized at 82 ± 2°C temperature for 30 minutes and acidity was adjusted to 1% with citric acid and preserved by chemical additives such as sodium benzoate, potassium meta-bisulphite (KMS) and potassium sorbet each at 0.2% and the KMS and sodium benzoate each at 0.066% in equal proportion. The pulp was stored at ambient temperature for three months and assessed for microbial and sensory qualities. The experiment was carried out in Completely Randomized Design (CRD) with five treatments. It was observed that the treatment in which pulp preserved with 0.2% KMS and stored in glass bottles scored maximum for overall acceptability and had negligible microbial count. This treatment was found statistically significant over the rest of treatments. It was further observed that the pulp was safe without spoilage up to 90 days.
 
Keywords
 
Mango, Pulp, Chemical preservatives, Sensory qualities, Storage, Shelf life
 
Introduction
 
Mango (Mangifera indica L.) is the most important fruit crop in tropical and sub-tropical regions of the world. Mango fruit is considered to be one of the best fruits in the world market because of its excellent flavor, attractive fragrance, beautiful color, delicious taste and health giving properties [1]. The pulp obtained from mango is increasingly used in beverage, dairy and confectionery industries, where fruit purees and concentrates are the major intermediates. Generally, partially processed mango, as an industrial viable raw material available round the year is mango juice as a base for the production of nectar and other beverages. Accordingly, the world’s total export volume of mango juice and pulp increased between 1999 and 2003 by 179% and 49% to 11,848 tons and 9510 tons, respectively, against the world production that has increased by 13% and 17%, since 1999 to 151,998 tons of mango juice and 925,263 tons of mango pulp in 2004 [2]. Indian export of fresh mango and mango pulp was 79060.88 MTS of worth Rs.141.94 crore and 156835.51 MTS of Rs.505.83 crore in 2006-07 respectively [3]. Mango pulp as a source of major and minor nutrients has not only justified its nutritional status but also recognized as nutraceutical on the basis of presence of carotene as an antioxidant. Mangoes with higher initial concentration of ß-carotene are found helpful for preventing the cancer [4].
 
The mango pulp is highly perishable in nature due to high moisture content hence, pulp is having shortest shelf life. Therefore, the efforts have been made in the present investigation in order to improve the shelf life of mango pulp by using the various safe preservatives and with their optimum concentrations.
 
Materials and Methods
 
Preparation of mango pulp
 
Uniformly ripened mangoes of Cv. Kesar were procured from fruit market of Aurangabad city. The fruits after washing, peeling and coring were subjected to slicing and feasible for macerating in an electric blender to get homogeneous pulp. Then pulp was pasteurized in a water bath at a temperature of 82 ± 2°C for 30 min to reduce the microbial load. The acidity was adjusted to 1.0% by addition of citric acid of commercial grade. The process flow chart for preparation of mango pulp is given in Figure 1.
 
Figure 1: Process flow chart for extraction of mango pulp.
 
Storage treatments of mango pulp
 
The pasteurized mango pulp was subjected to different predecided treatments and packed in 500 ml capacity glass bottles. The following treatments were finalized on the basis of efficacy of chemical preservatives. T0 = Control (without any chemical preservatives), T1 = 0.2% sodium benzoate, T2 = 0.2% potassium meta-bisulphite (KMS), T3 = 0.2% potassium sorbet, T4 = 0.066% each of KMS + Sodium benzoate.
 
The treated mango pulp was stored for a period of 3 months at ambient temperature (27-35°C). The pulp was assessed for microbial and sensory attributes at an interval of 15 days for a total storage period of 90 days.
 
Microbial and organoleptic evaluation of mango pulp
 
Total bacterial count (TBC): The pulp samples were analyzed for microbiological quality by considering the total plate count as evaluation index as described [5].
 
Sensory evaluation: Ready to serve mango beverages were prepared by using pulp as quality beverage (selected treatments) and evaluated by a panel of 11 semi trained judges for sensorial quality characteristics on a 9 point Hedonic scale like color, flavor and over all acceptability as described [6].
 
Statistical analyses: The data generated during triplicate experimentation were recorded and statistically analyzed on standard software using standard procedure for analysis of completely randomized design (CRD). The treatment means, their standard errors (SE) and critical differences (CD) at 5% level of significance were worked out for comparison of treatments [7]. Further, confidence intervals were also worked out.
 
Results and Discussion
 
Total bacterial count (TBC)
 
The data on total bacterial count of mango pulp presented in Table 1 indicate that there was significant increase in TBC (8.0-390 cfu/g) of mango pulp during storage without addition of any chemical preservatives. The pulp with KMS alone as chemical preservative or in combination thereof (potassium sorbet and sodium benzoate) reported TBC values at par followed by mango pulp preserved with sodium benzoate and potassium sorbet notifying TBC values significantly more as compared to former and also at par amongst themselves (T1-89, T3-88 cfu/g). On the basis of TBC count at the end of storage period, it could be concluded that Kesar mango pulp may be stored without alterations in nutritional parameters by using 0.2 % KMS as a chemical preservative. The results recorded in this investigation on microbial quality of mango pulp are in agreement with the findings reported [8,9].
 
Table 1: Effect of chemical preservatives on the total bacterial count (cfu/g) of mango pulp during storage*.
 
Organoleptic evaluation
 
 
The mango pulp treated with specified treatments and stored in glass containers for 90 days under ambient conditions was assessed for sensorial quality parameters at a frequent interval of time (15 days). The data on effect of various chemical treatments on quality parameters of mango pulp are presented in the respective tables.
 
Color: The organoleptic scores obtained for color are presented in Table 2. The sensory score for color of mango pulp without addition of chemical preservatives reported significantly decreased from 9 to 1.9 followed by pulp stored by using sodium benzoate and potassium sorbet during storage. The maximum score for color was observed in pulp with KMS (T2-6.5) followed by T4 (combination), while minimum score was recorded in control sample T0 (1.9). The highest score of mango pulp with KMS as a chemical preservative is associated with more effective control of browning retaining original color, whereas the reduction in color scores might be due to superimposition of intermediate color developed due to Maillard reaction accelerated between reducing sugars and amino acids during storage.
 
Table 2: Effect of chemical preservatives on organoleptic score for color of mango pulp during storage.
 
Flavor: The data pertaining to sensorial qualities with respect to flavor are presented in Table 3. The sensory scores for flavor of mango pulp without addition of chemical preservatives were significantly decreased from 8.8 to 1.4 followed by pulp stored by using sodium benzoate and potassium sorbet during storage. The maximum score was recorded in pulp preserved with KMS (T2-6.1) followed by T4 (Combination), whereas minimum score was recorded in the control sample without addition of chemical preservatives (T0-1.4).
 
Table 3: Effect of chemical preservatives on organoleptic score for flavor of mango pulp during storage.
 
Overall acceptability: The sensory scores obtained for overall acceptability of mango pulp are presented in Table 4. The score for overall acceptability of mango pulp without addition of chemical preservatives were significantly decreased from 8.9 to 1.6 during storage. The maximum score was recorded in pulp stored with KMS (T2-6.4) followed by T4 (Combination), whereas minimum score was recorded in the control sample without addition of any chemical preservatives (T0-1.6).
 
Table 4: Effect of chemical preservatives on overall acceptability of mango pulp during storage.
 
The pulp preserved with potassium meta-bisulphite either alone or in combination with other preservatives and stored in glass bottles retained maximum overall acceptability and negligible microbial count. Statistical analysis showed that storage intervals and treatments had a significant effect on total bacterial count and organoleptic scores for color, flavor and overall acceptability on the mango pulp during storage under the ambient conditions. The results are in conformity with the findings on preservation and storage of mango pulp [8,10-14].
 
Conclusion
 
It can be concluded that addition of 0.2% potassium meta-bisulphite (KMS) helps in controlling microbial growth and maintaining sensory characteristics of mango pulp packed in glass container and stored for 90 days.
 
 
References