A fast neutron mutant population in model rice variety KitaakeX and its use to facilitate forward and reverse genetics
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Copyright: © 2020 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The Kitaake variety (ssp. japonica), has emerged as a model for rice research. It is an extremely early flowering rice cultivar, easy to propagate, with good yield potential and eating quality. Here, we report the de novo genome sequencing and analysis of KitaakeX, a Kitaake variety carrying the XA21 immune receptor. Our Kita- akeX sequence assembly contains 377.6 Mb, consisting of 33 scaffolds (476 contigs) with a contig N50 of 1.4 Mb. The assembly is complemented with detailed gene annotations of 35,594 protein-coding genes. We identified 331,335 variations between KitaakeX and the reference genome Nipponbare (ssp. japonica), and 2,785,991 variations between KitaakeX and Zhenshan97 (ssp. indica).
