Research Article
Applications of Rat Brain Synaptic Vesicle Proteins for Sensitive and Specific Detection of Botulinum Neurotoxins
Manglesh Kumar Singh, Vinita Chauhan and Ram Kumar Dhaked*
Biotechnology Division, Defence Research & Development Establishment, Gwalior-474002, India
- *Corresponding Author:
- Ram Kumar Dhaked
Biotechnology Division
Defence Research & Development Establishment
Gwalior-474002, MP, India
Tel: 91-751-2233489
Fax: 91-751-2341148
E-mail: ramkumardhaked@hotmail.com
Received Date: April 09, 2012; Accepted Date: May 16, 2012; Published Date: May 23, 2012
Citation: Singh MK, Chauhan V, Dhaked RK (2012) Applications of Rat Brain Synaptic Vesicle Proteins for Sensitive and Specific Detection of Botulinum Neurotoxins. J Bioterr Biodef S2:008. doi:10.4172/2157-2526.S2-008
Copyright: © 2012 Singh MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
We propose here the application of synaptic vesicle proteins isolated from rat brain as a sole substrate for the specific endoproteinase activities of all seven serotypes of Botulinum Neurotoxin (BoNT/A to G). In this study, we used these proteins for evaluating endopeptidase and receptor binding activity for detecting BoNT/A by western blot and surface plasmon resonance with 6.25 pM and 0.22 fM limit of detection, respectively. Substrate and receptor present in the synaptic vesicle proteins are very robust and stable for more than 6 months to use in BoNT detection.
