Research Article
Biodegradation and Decolorization of Reactive Orange 16 by Nocardiopsis alba Soil Isolate
| Sugumar Shobana1* and Berla Thangam E2 | |
| 1Department of Bioinformatics, School of Bioengineering, SRM University, Kattankulathur 603203, India | |
| 2Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, India | |
| Corresponding Author : | Shobana Sugumar Department of Bioinformatics School of Bioengineering SRM University, Kattankulathur 603203, India E-mail: ksemaa@gmail.com |
| Received April 30, 2012; Accepted June 04, 2012; Published June 06, 2012 | |
| Citation: Shobana S, Thangam EB (2012) Biodegradation and Decolorization of Reactive Orange 16 by Nocardiopsis alba Soil Isolate. J Bioremed Biodeg 3:155. doi:10.4172/2155-6199.1000155 | |
| Copyright: © 2012 Shobana S, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
A bacterium identified as Nocardiopsis alba was isolated from acclimated sludge from a dyeing wastewater treatment plant. This strain rapidly decolorized Reactive orange16 (RO16) at 0.1% (w/v) concentration of both sucrose and peptone supplemented in Mineral Salt Medium (MSM) under static conditions at a temperature of 30°C with in 24 h with an initial dye concentration of 100 mg/L. The organism exhibited a remarkable color removal capability (95%) even at a high concentration of 1000 mg/L (RO16) dye within 24 h. The biodegradation products were analyzed by, FTIR spectroscopy and LC-MS analysis. The LC-MS analysis indicated the presence of 1-amino- 1-napthalene sulphonic acid in degraded product of the dye. The degraded product is less toxic to the growth of Vigna mungo seeds when compared to the non degraded dye.
