Research Article
Bioremediation on Effluents from Magnesite and Bauxite Mines using Thiobacillus Spp and Pseudomonas Spp
| Narayanan Mathiyazhagan and Devarajan Natarajan* | |
| Department of Biotechnology, Periyar University, Salem 636 011, Tamil Nadu, India | |
| Corresponding Author : | Dr. D. Natarajan Assistant professor Department of Biotechnology Periyar University Salem 636 011, India E-mail: mdnataraj@rediffmail.com |
| Received February 18, 2011; Accepted February 24, 2011; Published February 26, 2011 | |
| Citation: Mathiyazhagan N, Natarajan D (2011) Bioremediation on effluents from Magnesite and Bauxite mines using Thiobacillus Spp and Pseudomonas Spp. J Bioremed Biodegrad 2:115. doi: 10.4172/2155-6199.1000115 | |
| Copyright: © 2011 Mathiyazhagan N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
The present investigation was carried out to isolate metal tolerant bacterial species from the waste dump of magnesite and bauxite mines, Salem district, Tamil nadu. The result of primary and secondary screening showed two basophilic microbes were isolated from the effluents of mining industry. These two bacterial spp (namely P. aeruginosa and T. ferrooxidans ) were adapted for metal tolerance test, with different concentrations of (20 to 100μg/ ml -1 ) heavy metals (Zn, Mn, Cu, Cr and Hg). The P. aeruginosa and T. ferrooxidans showed high resistant to all metals. The minimum inhibitory concentration (MIC) of T. ferrooxidans tolerate upto 200 μg/ml -1 and P. aeruginosa posses MIC up to 150 μg/ml -1 concentration of heavy metals in order of Mn > Fe > Cu > Cr > Hg. These bacteria also expressed some antibiotic resistant potentiality was calculated by Antibiotic Resistant Index (ARI). The pre and post treated soils were analyzed by Atomic Absorbance Spectrophotometer (AAS) for heavy metals. The results of biosorption process showed the T . ferrooxidans reduced/ absorbed some heavy metals from mines (Cd, Ca, Zn, Cr, Mn & Pb) and P. aeruginosa was effectively absorbing the most of the metals than T. ferrooxidans . Both species are effectively absorbing Cd, Ca, Zn followed by Pb. This study include role of pH are also discussed. This study will be encourages further toxic metals remediation program and safe the environment in future.
