Research Article
Comparative Disposition Kinetics and Bioavailability of Ofloxacin after Extravascular Administration in Buffalo (Bubalus bubalis) Calves
| Pratishtha Sharma1, Ashok Gaur1, Manmeet Singh2 and Satish K Garg3* | |
| 1Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Rajasthan University of Veterinary and animal Sciences, Bikaner, India | |
| 2Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India | |
| 3Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, UP Pt. Deen Dayal Upadhyaya Pashu-Chikitsa Vigyan Vishwavidyalaya evam Go- Anusandhan Sansthan (DUVASU), Mathura, UP, India | |
| *Corresponding Author : | Satish Kumar Garg Department of Veterinary Pharmacology and Toxicology College of Veterinary Science and Animal Husbandry UP Pt. Deen Dayal Upadhyaya Pashu-Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan Mathura, UP, India Tel: 09456054995 E-mail: profsatish@gmail.com |
| Received January 10, 2016; Accepted January 27, 2016; Published January 30, 2016 | |
| Citation: Sharma P, Gaur G, Singh M, Garg SK (2016) Comparative Disposition Kinetics and Bioavailability of Ofloxacin after Extravascular Administration in Buffalo (Bubalus bubalis) Calves. J Pharmacokinet Exp Ther 1:103. doi:10.4172/jpet.1000103 | |
| Copyright: © 2016 Sharma P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
Abstract
Comparative disposition kinetics of ofloxacin was studied in buffalo calves following its Intramuscular (IM) and Subcutaneous (SC) administration @ 5 mg/kg body weight. Plasma ofloxacin concentrations were determined by microbial assay method using E. coli as test organism. Ofloxacin could be determined in plasma within 2.5 min of drug administration and peak levels of 2.37 ± 0.06 and 2.20 ± 0.02 μg/ml were observed at 0.75 and 1.0 h after IM and SC administration, respectively. Blood levels of around 0.25 μg/ml were observed for up to 24 h and pharmacokinetics was best described by two-compartment open model following both the routes. Absorption and elimination half life values were found to be 0.14 ± 0.02 and 22.88 ± 81.97 h after IM administration while 0.18 ± 0.04 and 28.25 ± 2.04 h after SC administration. The AUC values after IM and SC administration were calculated to be 24.99 ± 0.95 and 28.24 ± 0.65 μg.h/ml, respectively while respective bioavailability values were found to be 116.23 ± 8.03 and 107.10 ± 10.22 per cent indicating almost complete absorption of ofloxacin following administration by either of the routes. Based on the efficacy predictor values, ofloxacin be administered to buffalo calves @ 5 mg/kg by IM or SC route and be repeated at 24 h interval. However, for bacterial isolates requiring higher drug concentrations, ofloxacin be administered at 7.5 mg/kg and be repeated at 12 h interval. Further, preferential use of SC route over IM route is recommended for ofloxacin administration to buffalo calves.
