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Abstract

A Specific, Precise, Accurate, Robust and cost-effective UV spectroscopic and HPTLC methods were developed for simultaneous determination of Dapagliflozin (DAPA) and Metoprolol (METO) in their synthetic mixture. The developed methods proved to be simpler in procedure and produced more accurate results. The result of analysis was validated according to ICH Guidelines. This simple and precise method can be used of both drug in quality control laboratories. For HPTLC the drugs were separated by Camag Linomate 5 sample applicator with a 100-μL applicator syringe. Chromatography was performed on 10 cm × 10 cm aluminium TLC plates precoated with silica gel 60-F254. Plates were developed in a mobile phase consisting of toluene/ chloroform/ methanol/ glacial acetic acid (4.5/2/3/0.5, v/v/v/v). Developed plate subjected to densitometric measurement in absorbance mode at wavelength 235 nm using Camag TLC scanner. The % RSD Value was found for the validation parameter that indicate the preciseness of the proposed method and is applicable for routine analysis for quantitative determination of Dapagliflozin (DAPA) and Metoprolol (METO) in bulk as well as synthetic mixture.