Research Article
Development of Species-Specific Microsatellite Markers for Broomcorn Millet (Panicum miliaceum L.) via High-Throughput Sequencing
Min-Xuan Liu1, Yue Xu2, Tian-Yu Yang3, Zhi-Jun Qiao4, Rui-Yun Wang5, Yin-Yue Wang6and Ping Lu1*
1The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China
2School of Life Science, Jilin University, Changchun 130012, China
3Institute of Crop, Gansu Academy of Agricultural Sciences, Lanzhou 030000, Gansu, China
4Institute of Crop Genetic Resources, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi, China
5Shanxi Agricultural University, Taiyuan 030031, Shanxi, China
6Faculty of Life Science, Jilin Agricultural University, Changchun 130118, Jilin, China
- *Corresponding Author:
- Ping Lu
The National Key Facility for Crop Gene Resources
and Genetic Improvement/Institute of Crop Science
Chinese Academy of Agricultural Science, Beijing 100081, China
Tel: +86-010-6215-9962
E-mail: lupingcaas@163.com
Received date: July 18, 2017; Accepted date: July 24, 2017; Published date: July 27, 2017
Citation: Liu MX, Xu Y, Yang TY, Qiao ZJ, Wang RY, et al. (2017) Development of Species-Specific Microsatellite Markers for Broomcorn Millet (Panicum miliaceum L.) via High-Throughput Sequencing. Adv Crop Sci Tech 5: 297. doi:10.4172/2329-8863.1000297
Copyright: © 2017 Liu MX, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objectives: To discover and develop large-scale SSR markers of the P. miliaceum genome, which can be used in future genetic studies effectively.
Result: 223,894 putative SSR sequences were identified by next-generation sequencing. A total of 56,694 primer pairs were successfully designed and 240 primer pairs were randomly selected for effectiveness validation. The expected heterozygosity and observed heterozygosity varied from 0.0447 to 0.7713 and from 0 to 0.9545, respectively and the mean of Shannon information index (I) was 0.7254. A UPGMA dendrogram indicated the high quality and effectiveness of these novel genomic SSR markers developed via next-generation sequencing technology.
Conclusion: A large repertoire of SSR markers were successfully developed by next-generation sequencing of the P. miliaceum genome which will be useful for the construction of genetic linkage maps, the identification of QTLs, and marker-assisted selection breeding.
