Double-Muscled Phenotype in Mutant Sheep Directed by the CRISPRCas9 System
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Copyright: © 2018 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Myostatin (MSTN) is a well-known negative regulator of muscle growth. The double-muscled sheep caused by natural loss-of-function mutations of MSTN have very strong skeletal muscle. In this study, our results demonstrate the successful generation of MSTN mutant sheep via specific targeting of an exon 1 site using Cas9 technology.
