Eco-Friendly Biodegradation of Plastics by Intuitive Bacterial Strains from Polyethylene Polluted Sites and Optimizing its Culture Conditions by Response Surface Methodology (Rsm)

Hemapriya Janarthanam; Reshma Girirajan; Rajalakshmi Balaji; Jeevitha Prakasam; Vijayanand Selvaraj

Eco-Friendly Biodegradation of Plastics by Intuitive Bacterial Strains from Polyethylene Polluted Sites and Optimizing its Culture Conditions by Response Surface Methodology (Rsm)

Hemapriya Janarthanam¹^*, Reshma Girirajan², Rajalakshmi Balaji³, Jeevitha Prakasam⁴ and Vijayanand Selvaraj⁵: ¹Department of Microbiology, DKM College for Women (Autonomous), Vellore, Tamilnadu, India; ²Department of Microbiology, DKM College for Women (Autonomous), Vellore, Tamilnadu, India; ³Department of Microbiology, DKM College for Women (Autonomous), Vellore, Tamilnadu, India; ⁴Department of Microbiology, DKM College for Women (Autonomous), Vellore, Tamilnadu, India; ⁵Departments of Biotechnology, Thiruvalluvar University, Serkkadu, Tamilnadu, India

^*Corresponding Author : Hemapriya Janarthanam, Department of Microbiology, DKM College for Women (Autonomous), Vellore, Tamilnadu, India, Email: bentresearchgroup2018@gmail.com

Received Date: Jan 02, 2023 / Accepted Date: Jan 30, 2023 / Published Date: Jan 30, 2023

Abstract

The aim of this study was to investigate the biodegradation of polyethylene by two bacterial strains isolated from polyethylene contaminated sites. This work revealed that the soil of areas contaminated with polyethylene is a good source of bacteria capable of decomposing plastic materials. The biodegradability of the two bacterial strains was evaluated by performing invasion studies, SEM analysis and Sturm assay. Strains capable of metabolizing complex plastics. The plastic samples tested in this study were plastic cups, plastic bags and milk carton lids. Among the samples, milk pods were shown to be optimally degraded (24.40%) by Pseudomonas sp. and plastic bags were found to have optimal degradability (20.40%) by Bacillus sp. after 45 days of incubation. In this study, the important variables affecting PE depletion of the contaminated sites by isolates B1 and B7 and the optimized values were determined by optimizing the values. Values were filtered using the central composite design (CCD) in the Response Surface Methodology.

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Citation: Janarthanam H, Girirajan R, Balaji R, Prakasam J, Selvaraj V (2023) Eco-Friendly Biodegradation of Plastics by Intuitive Bacterial Strains from Polyethylene Polluted Sites and Optimizing its Culture Conditions by Response Surface Methodology (Rsm). J Bioremediat Biodegrad, 14: 549.

Copyright: © 2023 Janarthanam H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Journal of Bioremediation & Biodegradation
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