Effect of Seed Inoculation with Actinomycetes and Rhizobium Isolated from Indigenous Soybean and Rhizosphere on Nitrogen Fixation, Growth, and Yield of Soybean
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Abstract
The present study was initiated to determine whether isolates from soil and of soybean plants can express nitrogenase activity when grown in the absence of plant hosts. The study was conducted to answer the question“can benefit gained by” the interaction between Actinomycetes and Rhizobium symbiosis with a legume. Thirty-five isolates identified as Rhizobium and twenty-one Action my cetes were isolated from the rhizosphere of soybean plants and identified by morphological character, biochemical content identified. Fifty-six isolates were tested for their capabilities of N2 fixation and siderophore production. The isolated rhizobacteria were grown in N-free media, and twelve of them showed good growth on the Burk’s N-free media. Almost all strains produced siderophores; however, the production level was very low, and only the strain AK 10 released considerable amounts of this metabolite. One strain of Actinomycetes was selected to test the interactions with Rhizobium. Co inoculation of Actinomycetes and Rhizobium produced synergic benefits on plant growth and get protection from the production of siderophore.
