Research Article
Effects of 6-Benzyl aminopurine and Kinetin on In Vitro Shoot Multiplication of Sugarcane (Saccharum officinarum L.) Varieties
| Belay Tolera1*, Mulugeta Diro2 and Derbew Belew3 | ||
| 1Ethiopian Sugar Corporation, Research and Training Division, Variety Development Directorate, Biotechnology Research Team, Wonji Research Center, Wonji, Ethiopia | ||
| 2Capacity Building for Scaling up of Evidence-based Best Practices in Agricultural Production in Ethiopia (CASCAPE) Project, Addis Ababa, Ethiopia | ||
| 3Jimma University, College of Agriculture and Veterinary Medicine, Jimma, Ethiopia | ||
| Corresponding Author : | Dr. Belay T Ethiopian Sugar Corporation Research and Training Division, Ethiopia Tel: +251-910181644 E-mail: belaytolera@yahoo.com |
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| Received March 03, 2014; Accepted April 18, 2014; Published April 20, 2014 | ||
| Citation: Tolera B, Diro M, Belew D (2014) Effects of 6-Benzyl aminopurine and Kinetin on In Vitro Shoot Multiplication of Sugarcane (Saccharum officinarum L.) Varieties. Adv Crop Sci Tech 2:129. doi: | ||
| Copyright: © 2014 Tolera B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||
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Abstract
In vitro shoot multiplication of two commercial sugarcane varieties grown in Ethiopian sugar estates initiated using shoot tip explants was carried out with the objective to assess the multiplication responses of the sugarcane varieties (B41-227 and N14) under five levels of 6-benzylaminopurine (0, 0.5, 1, 1.5 and 2 mgL-1) and kinetin (0, 0.1, 0.25, 0.5, and 1 mgL-1) in a completely randomized design with 5x5x2 factorial treatment combinations arrangements. Analysis of variance (ANOVA) showed that the interaction effects of 6-benzylaminopurine, kinetin and the sugarcane genotypes on number of shoots per explant, average shoot length and number of leaves per shoot was very highly significant (P < 0.0001). Murashige and Skoog (MS) medium fortified with 1 mgL-1 6- benzylaminopurine (BAP) and 0.5 mgL-1 kinetin for B41-227; and 2 mgL-1 6-benzylaminopurine (BAP) without kinetin for N14 were found to give optimum shoot proliferation results. On these medium, B41-227 gave 34 ± 1.54 shoots per explant with 6.95 ± 0.01cm average shoot length and 12 ± 0.17 leaves per shoot while N14 produced 21 ± 0.58 shoots per explant with 5.63 ± 0.01 cm average shoot length and 5.4 ± 00 leaves per shoot after 30 days of culture transfer to multiplication medium. Thus, the optimized protocol is useful for rejuvenation, rapid In vitro propagation and production of large quantity quality sugarcane planting materials and hence help minimizes the major limitations hampering sugarcane production in the Ethiopian sugar Estates.
