Research Article
Effects of Fe Deficiency on Organic Acid Metabolism in Pisum sativum Roots
| Nahida Jelali1*, Marc El Beyrouthy2l, Marta Dell’orto3, Mohamed Gharsallil and Wissem Mnif4 | ||
| 1Laboratoire Plant Adaptation to Abiotic Stress, Centre for Biotechnology, Borj-Cedria Technopark (CBBC), BP 901, 2050 Hammam-Lif, Tunisia | ||
| 2Faculty of Agricultural and Food Sciences, Holy Spirit University of Kaslik, P.O. Box 446, Jounieh, Lebanon | ||
| 3Dipartimento of Agricultural and Environmental Sciences-Production, Land, Agroenergia, University of Milan, Via Celoria 2, I-20133 Milan, Italy | ||
| 4LR11-ES31 Biotechnology and Bio-Enhancement of Geo Resources, Higher Institute of Biotechnology of Sidi Thabet Sidi Thabet BiotechPole, 2020, Tunisia Manouba University, Tunisia | ||
| Corresponding Author : | Dr. Nahida Jelali Laboratoire Plant Adaptation to Abiotic Stress Centre for Biotechnology, Borj-Cedria Technopark (CBBC) BP 901, 2050 Hammam-Lif, Tunisia E-mail: nahidajelali@yahoo.fr |
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| Received January 31, 2013; Accepted February 28, 2013; Published March 06, 2013 | ||
| Citation: Jelali N, Beyrouthy MEl, Dell’orto M, Gharsalli M, Mnif W (2013) Effects of Fe Deficiency on Organic Acid Metabolism in Pisum sativum Roots. Adv Crop Sci Tech 1:102. doi:10.4172/2329-8863.1000102 | ||
| Copyright: © 2013 Jelali N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||
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Abstract
Iron deficiency induces several responses to iron shortage in plants. Metabolic changes occur to sustain the increased iron uptake capacity of Fe-deficient plants. The aim of this work was to investigate the impact of Fe deficiency on the organic acid metabolism in Pisum sativum roots. For this purpose, seedlings of Pisum sativum (cv. Douce) were grown under controlled conditions, in the presence of iron sufficient (C) or deficient (D) mediums. Our results showed that PEPC activity increased by 290% in root extracts of Fe deficient plants, compared to the control. Citrate concentration increased in Fe deficient Pisum roots (114% of the control). As well, MDH, CS and ICDH activities showed a marked increase in roots subjected to D treatment. However, the extent of stimulation was especially important for MDH and ICDH activities (99% and 150% of the control, respectively). These data suggest that the capacity of Douce cultivar to increase organic acid metabolism enzyme activities under iron deficiency is related to its better Fe-use efficiency, which indicate the tolerance level of this cultivar to iron chlorosis.
