Research Article
Enzymatic Potential of Mucor inaequisporus for Naringin Biotransformation, Accessed by Fractional Factorial Design and Mass Spectrometry Analysis
Enzo Monte Canedo, Taicia Pacheco Fill*, Edenir Rodrigues Pereira-Filho and Edson Rodrigues-FilhoChemistry Department, Universidade Federal of São Carlos, São Carlos, Brazil
- *Corresponding Author:
- Taicia Pacheco Fill
Chemistry Department, Universidade Federal de São Carlos CP 676
13.565-905, São Carlos, SP, Brazil
Tel: +55-16-3351-8053
Fax: +55-16-3351-8350
E-mail: taicia@gmail.com
Received date: October 4, 2014; Accepted date: October 27, 2014; Published date: October 31, 2014
Citation: Canedo EM, Fill TP, Pereira-Filho ER, Rodrigues-Filho E (2014) Enzymatic Potential of Mucor inaequisporus for Naringin Biotransformation, Accessed by Fractional Factorial Design and Mass Spectrometry Analysis. J Anal Bioanal Techniques S6:006. doi: 10.4172/2155-9872.S6-006
Copyright: © 2014 Canedo EM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Whole cells of Mucor inaequisporus were used for biotransformation of the flavonoid naringin, as a green method for structural diversification of this class of natural product. The metabolism of the fungus was challenged against naringin under several culturing conditions, using statistical optimization. Eight parameters were evaluated comprising type and concentration of carbon source, substrate concentration, substrate addition time, shaking, luminosity, temperature and extraction time. Sixteen different culture conditions were tested in this screening. After LC-MS analysis, several biotransformation products were identified and naringenin was identified as the major product. Eleven biotransformation products were detected in Ft-HRMS analyses and identified as naringenin as major compound, 4’-methoxynaringenin, 4’-methoxynaringin, rhoifolin, apigenin, methoxyapigenin, acetylated naringin, and also two biflavonoids and two triflavonoids. Ft-HRMSn showed to be a powerful technique for structural elucidation of flavonoid biotransformation products. The fungus was capable to perform deglycosylation, reduction and O-methylation reactions at this substrate indicating good enzymatic potential, since these reactions weren’t observed before for this fungus-genus.
