Review Article
Extraction of Proteases from Medicinal Plants and their Potential as Anti- Viral Targets
Amit Gupta1, Ankit P Shah2 and Sushama R Chaphalkar11Department of Immunology and Virology, Vidya Pratishthan’s School of Biotechnology (VSBT, Research centre affiliated to Savitribai Phule Pune University), Baramati, Maharashtra, India
2MAEER’s Maharashtra Institute of Pharmacy (MIT), Pune, India
- Corresponding Author:
- Amit Gupta
Department of Immunology and Virology
Vidya Pratishthan’s School of Biotechnology (VSBT)
Baramati, Baramati, Maharashtra, India
E-mail: amitvsbt@gmail.com, amitgupta@vsbt.res.in
Received Date: May 05, 2016; Accepted Date: May 13, 2016; Published Date: May 20, 2016
Citation: Gupta A, Shah AP, Chaphalkar SR (2016) Extraction of Proteases from Medicinal Plants and their Potential as Anti-Viral Targets. J Biotechnol Biomater 6:228. doi:10.4172/2155-952X.1000228
Copyright: © 2016 Gupta A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Medicinal plants especially leaves are used in traditional medicine for rapid anti-viral therapy against infectious diseases. Protease, a potential candidate in medicinal plants is not so for studied in leaves. So an attempt was made to determine the protease activity of various medicinal plants especially leaves. Buffers of different pH range were used for extraction of the leaves to identify the best buffer for extraction of protease. Firstly, protein from fresh plant leaves of these medicinal plants were determined and then evaluated its protease activity using crude enzyme of protein (leaves) against specific protein antigen i.e. Bovine serum albumin (BSA). Thereafter, exposure of these proteases (acid or basic) on virally infected human whole blood samples determined through flow cytometry. The results showed that protease at particular pH of PBS buffer range of these medicinal plant leaves on virally infected human whole blood samples showed anti-viral activity.
