Research Article
Ex-Vivo Trace Assay Using Silver-doped Microfilm Electrode
Suw Young Ly* and Juo Yang KimBiosensor Research Institute, Seoul National University of Science and Technology, 172, gong reung 2-dong, Nowon gu, Seoul, South Korea
- *Corresponding Author:
- Suw Young Ly
Biosensor Research Institute
Seoul National University of Science and Technology
172, gong reung 2-dong, Nowongu
Seoul, South Korea 139-743
Tel: +82 2 970 6691
Fax: +82 2 973 9149
E-mail: suwyoung@snut.ac.kr
Received April 20, 2011; Accepted July 01, 2011; Published July 20, 2011
Citation: Young Ly S, Kim JY (2011) Ex-Vivo Trace Assay Using Silver-doped Microfilm Electrode. J Addict Res Ther 2:113. doi:10.4172/2155-6105.1000113
Copyright: © 2011 Young Ly S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Trace lead is a dangerous environmental chemical that is associated with increased cancer risk. In this study, assay was conducted in ex-vivo mouse fluid, using a silver-doped microfilm electrode (SDE). The analytical working range was from 20 to 160 mg/L. Under optimum conditions, the micro range improved to 5-65μg/L while the detection limit was 5μg/L. Under a 10.0-mg/L Pb constant, the 10-time statistic was R 2 =9.795. This result was applied to mice ordure using SDE via cyclic and square-wave stripping voltammetry. As a result, 27.6μg/L Pd(II) was detected in the mice ordure solution with a precision value of ±12.5%. The techniques developed in this study using a probe that did not require pretreatment and expensive equipment can be applied in medical diagnosis.
