Research Article
GC-MS Analysis of Phytochemicals and Simultaneous Determination of Flavonoids in Amaranthus caudatus (Sirukeerai) by RP-HPLC
Paranthaman R1*, Praveen kumar P2 and Kumaravel S3
1Technical Assistant, Indian Institute of Crop Processing Technology, Ministry of Food Processing Industries, Govt. of India, Thanjavur, India
2Senior Research Fellow, Indian Institute of Crop Processing Technology, Ministry of Food Processing Industries, Govt. of India, Thanjavur, India
3Scientist & Head i/c, Indian Institute of Crop Processing Technology, Ministry of Food Processing Industries, Govt. of India, Thanjavur, India
- *Corresponding Author:
- R. Paranthaman
Technical Assistant
Indian Institute of Crop Processing Technology
Ministry of Food Processing Industries, Govt. of India
Thanjavur-613 005 (TamilNadu), India
Tel: 04362226676
E-mail: paranthhu@gmail.com
Received date: July 10, 2012; Accepted date: September 21, 2012; Published date: September 27, 2012
Citation: Paranthaman R, Praveen kumar P, Kumaravel S (2012) GC-MS Analysis of Phytochemicals and Simultaneous Determination of Flavonoids in Amaranthus caudatus (Sirukeerai) by RP-HPLC. J Anal Bioanal Tech 3:147. doi: 10.4172/2155-9872.1000147
Copyright: © 2012 Paranthaman R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
A sensitive and selective high performance liquid chromatography method (HPLC) for simultaneous analysis of following five flavonoids like Gallic acid (GA), Caffeic acid (CA), Rutin (RU), Ferulic acid (FA) and Quercetin (QU) in Amaranthus caudatus (Sirukeerai) leaves. The results demonstrated that the Amaranthus caudatus leaves were separately extracted and analyzed using RP HPLC method. The contents of Gallic acid (0.083 μg/gm), Caffeic acid (0.004 μg/gm), Rutin (0.019 μg/gm), Quercetin (0.001 μg/gm) and Ferulic acid (0.001 μg/gm) in Amaranthus caudatus leaves. The GC-MS study also carried out and it showed the presence of phytochemicals. Hence these plants extracts with the property of bioavailability and retention of certain minerals by polyphenolic compounds can be recommended for their use as an alternative anti-infective agent in natural medicine for the treatment of infectious diseases.
