Research Article
Genetic Diversity Analysis of Urtica Parviflora in Uttarakhand Himalayas by Rapid Marker
Sandeep Sharma1*, Syed Atif Ali2, Alok Khare3 and Tapan Kumar Nailwal21National Bureau of Plant Genetic Resources (ICAR), Pusa Campus New Delhi, India
2Departments of Biotechnology, Kumaun University, Nainital, Uttarakhand, India
3Departments of Botany, Bareilly College, Bareilly, India
- Corresponding Author:
- Sandeep Sharma
Division of Plant Physiology
IARI New Delhi 110012, India
Tel: +919557285687
E-mail: saan.sharma07@gmail.com
Received date: January 30, 2015; Accepted date: May 25, 2015; Published date: June 01, 2015
Citation: Sharma S, Ali SA, Khare A, Nailwal TK (2015) Genetic Diversity Analysis of Urtica Parviflora in Uttarakhand Himalayas by Rapid Marker. J Biotechnol Biomater 5:183. doi:10.4172/2155-952X.1000183
Copyright: © 2015 Sharma S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Urtica parviflora is herbaceous perennial medicinal plant distributed in Himalayan region from 400m to 3000m amsl altitude range. In the present study, five genotypes of U. parviflora were collected from different altitudes having variability in morphological traits. This genotype was subjected to Random Amplified Polymorphic DNA (RAPD) marker analysis to assess the genetic diversity at molecular level and develop molecular marker for their identification. Fairly rich genetic diversity was found through RAPD marker analysis. A total 66 scorable bands were produced in five samples with 8 primers. Out of 66 bands, 43 bands were found to be polymorphic i.e., 65.15% polymorphism. The dendrogram of samples shows three major clusters. The samples of similar altitudes were found to be present in one cluster.
