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Abstract

Objective: Helicobacter pylori a bacteria that colonises the human stomach, is the most prevalent organism that infects people worldwide. Additionally, this bacterium has been found in various natural niches outside of the stomach, like the mouth and water. The outcomes of H. pylori identification in extra-gastric ecological niche, however, are debatable. One of the primary challenges in presenting strong evidence appears to be improving the sensitivity and specificity of the detection systems. The purpose of this study was to use a new nucleic acid detection technique called Loop-mediated Isothermal Amplification along with an analytically sensitive and specific Polymerase Chain Reaction to identify the presence of this bacterium in dental plaque samples.
Design: 45 participants were engaged in a descriptive cross-sectional study, and samples of dental plaque were taken with a sterile periodontal curette from at least two tooth surfaces. DNA was isolated from the samples, and PCR and LAMP procedures were used to detect the presence of H. pylori.
Results: Using PCR, LAMP, and positive for both tests, the prevalence of H. pylori identification in dental plaque samples was 44%, 66.67%, and 77.78%, respectively.
Conclusion: Participants’ dental plaque samples contained high levels of H. pylori, which is consistent with the high prevalence of these germs in the general population. One of the highest reported rates ever is this one. The findings show that oral-to-oral transmission and one of the primary causes of re-infection can both be caused by dental plaque.