Research Article
Isolation and Characterization of Octadecanoic Acid from The Ethyl Acetate Root Extract of Trigonella foneum graecum L. by Using Hydroponics Method
Sudharsan1*, R Saravanan1, A Shanmugam1, S Vairamani1, R Mohan kumar2, S Menaga2 and N Ramesh3
1Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai-608 502, Tamil Nadu, India
2Astagiri Herbal Research Foundation, Chennai-600 047, Tamil Nadu, India
3Deparment of Biotechnology, JJ College of Arts and Science, Pudhukottai, Tamil Nadu, India
- *Corresponding Author:
- R Saravanan
CAS in Marine Biology
Faculty of Marine Science
Annamalai University
Parangipettai-608 502
Tamil Nadu, India
E-mail: saran_prp@yahoo.com
Received Date: November 19, 2010; Accepted Date: January 29, 2011; Published Date: January 31, 2011
Citation: Sudharsan S, Saravanan R, Shanmugam A, Vairamani S, Mohan kumar R, et al. (2011) Isolation and Characterization of Octadecanoic Acid from The Ethyl Acetate Root Extract of Trigonella foneum graecum L. by Using Hydroponics Method. J Bioterr Biodef 2:105. doi: 10.4172/2157-2526.1000105
Copyright: © 2011 Sudharsan S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The octadecanoic acid (OA), methyl ester was isolated and characterized from root of fenugreek (T. foenumgraecum) using ethyl acetate (EAC) in hydroponics method. The OA was characterized by TLC & HPTLC and purified by HPLC. The structure and molecular weight (298) of OA was confirmed by GC-MS. The Rf value of sample (peak 4) was matched with standard. OA has strong anti-bacterial activity in B. subitlis and P. aeruginousa, where as weak activity in S. aureus and E. coli. Among the tested concentration of in vitro anti-oxidant activity of OA was maximum in 40µl and minimum in 20µl.
