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Abstract

Bacterial and fungal pollutants generate a main problem in plant tissue culture research laboratory. This study meant at exploring the sources of microbial contamination in tissue culture laboratory. The bacterial pollutants comprise Pseudomonas fluorescents’, Escherichia coli, Proteus sp, Micrococcus sp, Streptococcus pneumoniae, Staphylococcus aureus, Bacillus cereus, Bacillus subtilis and Corynebacterium sp. Although Mushrooms separates were Aspergillus niger, Aspergillus fumigatus, Penicillium sp. Yeast, Fusarium sp. The cruel incidence of pollutants in the vegetable tissue culture laboratory was deliberate. The greatest principal bacterial pollutants remained Micrococcus sp., Staphylococcus aureus and Bacillus cereus and greatest controlling mycological pollutants remained Aspergillus sp., Penicillium sp. and Yeast. Highest mean occurrence was recorded for fungal contaminants. The research laboratory walls and grounds likewise harbored greatest of the polluting microorganisms. The monthly occurrence of contaminants, its source and elimination or prevention of contaminants was the key objectives of the work. Fumigation is an actual technique of sterilization of cell culture laboratories. Hence in this work we also examined the effectiveness of fumigation.

Keywords: Microorganisms, Tissue Culture, Sterilization.