Research Article
STANDARDIZATION OF IN VITRO STERILIZATION AND CALLUS INDUCTION PROTOCOL FOR LEAF EXPLANTS OF ANCHOTE : COCCINIA ABYSSINICA
Abstract
The present study was conducted to develop efficient sterilization and callus induction protocols of Anchote leaf explants. Anchote (Coccinia abyssinica) roots were collected from West Ethiopia transported to Kenya where they were grown in the net greenhouse in Juja. Leaves explants were harvested from the seedlings and used for both sterilization and callus induction protocols. Young leaf explants were selected from healthy plants and subjected sterilization using 5 and 10 % commercial bleach of( NaOCl (3.85%) /(JIK®) )at 5 ,10 and 15 minutes intervals. Maximum clean survival explants were obtained (82.5±0.5) at 5%NaOC with 10Minutes) . Best maximum callus induction(80±2) was achieved from the combination of 5μm(BAP+2,4-D).
