Research Article
Study of Breath Acetone in a Rat Mode of 126 Rats with Type 1 Diabetes
Zhennan Wang1, Meixiu Sun1, Xiaomeng Zhao1, Chenyu Jiang1, Yingxin Li1 and Chuji Wang1,2*1Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
2Department of Physics and Astronomy, Mississippi State University, MS 39759, USA
- *Corresponding Author:
- Chuji Wang
Department of Physics and Astronomy
Mississippi State University, MS 39759, USA
Tel: +16623259455
Fax: +16623258898
E-mail: cw175@msstate.edu
Received date: December 28, 2016; Accepted date: January 18, 2017; Published date: January 24, 2017
Citation: Wang Z, Sun M, Zhao X, Jiang C, Li Y, et al. (2017) Study of Breath Acetone in a Rat Mode of 126 Rats with Type 1 Diabetes. J Anal Bioanal Tech 8:344. doi: 10.4172/2155-9872.1000344
Copyright: © 2017 Wang Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
We investigate correlations of breath acetone with blood glucose (BG) and blood beta-hydroxybutyrate (BHB) in a rat model of 126 streptozotocin-induced Type 1 diabetic (T1D) and 32 healthy rats, and evaluate the role of breath acetone analysis as a non-invasive alternative in diabetes and management. We conducted breath acetone analysis using a T1D rat model under various conditions, including fasting and insulin-treated. Breath acetone concentrations were measured using a cavity ringdown breath acetone analyzer, LaserBreath-001. The results showed that the breath acetone, BG, and BHB in the T1D rat group were significantly different from those in the healthy group (P<0.05). Significant positive relationships between breath acetone and blood BHB existed in both T1D and healthy groups, as well as in a group of T1D rats under fasting condition and insulin treatment. When the BG concentrations were grouped as follows: 16.5-24.4, 24.5-27.7, and 27.8-41.7 mmol/L, a moderate negative correlation between breath acetone and BG was observed in the T1D group. Furthermore, the accuracy of screening using breath acetone was analyzed using the receiver operating characteristic (ROC) analysis method and results showed that the accuracy is low when the area under ROC curve (AUC) is <0.6425.
