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Abstract

In vitro medication screening is a critical step in drug discovery and development, providing valuable insights into drug efficacy and toxicity before progressing to costly and time-consuming in vivo studies. However, the success of in vitro screening assays depends heavily on the cellular microenvironment, or "cell climate," which encompasses factors such as temperature, pH, oxygen levels, and nutrient availability. In this study, we investigate the effect of cell climate on in vitro medication screening outcomes. Using various cell culture conditions mimicking physiological and pathological states, we evaluate the impact of temperature, pH, and oxygen tension on drug response and toxicity profiles. Our results reveal significant alterations in drug sensitivity and toxicity depending on the cell climate, highlighting the importance of carefully controlling these parameters in in vitro screening assays. By optimizing cell culture conditions to better reflect the in vivo microenvironment, we can enhance the predictive power of in vitro medication screening and accelerate the discovery of safe and effective therapeutics.