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Abstract

The water frameworks inside a dental unit are known to be defiled with a multi-realm biofilm enveloping microorganisms, organisms, infections, and protozoa. These microorganisms’ aerosolization may pose a health risk to the patient and dental staff alike. The effectiveness of products used to disinfect dental units against amoebas is poorly understood. An in-vitro multi-kingdom dental unit water system (DUWS) biofilm was subjected to four distinct treatment protocols, each containing Oxygenal, a product containing hydrogen peroxide (H2O2). Heterotrophic plate counts, the bacterial 16S rDNA gene load, the fungal 18S rDNA gene load, and the number of genomic units for Legionella spp. were used to measure treatment efficacy over time. the single adaptable cell Vermamoeba vermiformis. According to the findings, it is necessary to treat the DUWS on a daily basis with a low dose of H2O2 (0.02% for 5 hours) and a weekly shock dose (0.25% H2O2, 30 minutes) to bring the heterotrophic plate count of a severely contaminated DUWS down to below 100 CFU mL-1. The total amount of bacterial 16S rDNA gene, Legionella spp., can be statistically significantly reduced by daily treatment with only a low dose of hydrogen peroxide. and the load of Vermamoeba vermiformis (p 0.005). The detachment of the trophozoite form of this amoeba from the DUWS biofilm, thereby removing the amoeba from the system, is also demonstrated, despite the fact that hydrogen peroxide does not kill the trophozoite or the cysts of V. vermiformis.