Biological Variability of SARS-CoV-2 RT-PCR Nasopharyngeal Swab Test
Received Date: Dec 17, 2020 / Accepted Date: Dec 24, 2020 / Published Date: Jan 07, 2021
Abstract
We studied the variability among viral target Cycle Thresholds (Cts) of SARS-CoV-2 RT-PCR collected with conventional and 3D printed nasopharyngeal swabs from separate nostrils at the same time by the same investigator. There was far greater variation in the viral copy number than could be explained by the amount of material collected, as measured by the Cts of the RNaseP human Internal Control Gene (IC). We conclude that there are underlying biological differences in the distribution of the virus within the nasopharynx and nasal passages and that this communication encourages further study.
Keywords: COVID-19; Nasopharynx (VI); Printing, Three- Dimensional; Polymerase Chain Reaction; Severe Acute Respiratory Syndrome Coronavirus 2; Ribonuclease P
Citation: Rand KH, Cherabuddi K, Lauzardo M, Beal SG, Harris N, et al. (2021) Biological Variability of SARS-CoV-2 RT-PCR Nasopharyngeal Swab Tests. J Infect Dis Ther 9:449. Doi: 10.4172/2332-0877.1000449
Copyright: © 2021 Rand KH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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