Research Article
Expression of the Sortilin Gene in Cultured Human Keratinocytes Increases in a Glucose-free Medium
| Kyoichi Matsuzaki1,2*, Miyuki Tomioka3, Yuichi Watabe3 | ||
| 1 Director, Department of Plastic and Reconstructive Surgery, Kawasaki Municipal Tama Hospital, Japan | ||
| 2 Associate Professor, Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Japan | ||
| 3 Researcher, Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Japan | ||
| Corresponding Author : | Kyoichi Matsuzaki Director, Department of Plastic and Reconstructive Surgery Kawasaki Municipal Tama Hospital 1-30-37 Shukugawara, Tama-Ku Kawasaki 214-8525 Japan Tel: +81-44-933-8111 Fax: +81-44-933-8362 E-mail: k4matsu@marianna-u.ac.jp |
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| Received December 05, 2013; Accepted January 20, 2014; Published January 25, 2014 | ||
| Citation: Matsuzaki K, Tomioka M, Watabe Y (2014) Expression of the Sortilin Gene in Cultured Human Keratinocytes Increases in a Glucose-free Medium. Clin Res Foot Ankle S3:004. doi:10.4172/2329-910X.S3-004 | ||
| Copyright: © 2014 Matsuzaki K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||
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Abstract
Introduction: When proNGF binds to sortilin and p75, the cell death signal is transduced into the cell. This study examined how sortilin gene expression changes in cultured human epidermal cells depending on the glucose concentration in the culture medium. The results might help elucidate if sortilin in epidermal cells is involved in the delay of wound healing in diabetes.
Materials and methods: Epidermal cells were grown in a serum-free, glucose-free culture medium for 24 h. The new medium was prepared with a glucose concentration of 0, 20, 40, 60, 100, 200, 400, or 800 mg/dL. The cells were grown in each medium for an additional 24, 48, and 72 h. At each culture time, the sortilin gene expression level at 100 mg/dL glucose was compared with the expression levels at other glucose concentrations.
Results and discussion: When the cells were grown in the new medium for 24 h, the sortilin gene expression level at 100 mg/dL glucose did not differ significantly from the expression levels at other glucose concentrations. At 48 h of culture, only the sortilin gene expression level at 0 mg/dL glucose was significantly higher than the expression level at 100 mg/dL glucose (p=0.002). At 72 h of culture, only the expression level at 0 mg/dL glucose was significantly higher than the expression level at 100 mg/dL glucose (p<0.001). The results suggest that the sortilin gene levels of epidermal cells are not clinically problematic at low glucose concentrations, just as at high glucose concentrations, provided the glucose levels are sufficient to maintain biological activities.
