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Abstract

Osteoarthritis of the Ankle, or Ankle OA, is an irreversible condition that causes bone deformity, degeneration of articular cartilage, joint pain and limited joint movement. Clinical signs of Ankle OA are reduced when cultured adipose stem cells (ADSCs) are injected into the Ankle joint space. However, the impact of SF filling the joint space of injected ADSCs is still unknown. By adding SFs from Ankle OA patients to treatment-prepared cultured ADSCs, joint space was simulated in this study. Addition of SF improved the survival rate of ADSCs. DNA microarray-based gene expression profiles of SF-treated ADSCs revealed that a number of survival-related genes were altered. We focused on FOSL1, which is involved in both ADSC therapeutic efficacy and cancer stem cell survival and proliferation. Upregulation of FOSL1 mRNA and protein expression was confirmed by RT-PCR and Western blot, respectively. Then, we knocked out FOSL1 in ADSCs with siRNA. A decrease in cell viability was observed, suggesting that ADSCs cannot survive without FOSL1. Based on these findings, SF appears to increase cell viability by increasing her FOSL1 expression in her ADSCs. ADSC therapeutic efficacy using cultured ADSCs can be enhanced if a more conducive environment for upregulation of FOSL1 expression in ADSCs can be created.