Research Article
speB Genosensor for Rapid Detection of Streptococcus pyogenes Causing Damage of Heart Valves in Human
Abstract
speB Genosensor for early detection of Streptococcus pyogenes in human throat swab samples were fabricated using screen printed modified gold electrode. The electrochemical changes before and after hybridization was measured using cyclic voltammetry (CV) in the presence of redox indicator. The sensitivity of the genosensor was 80(μA/cm2)/ng and lower limit of detection (LOD) was 0.10 ng/6μl with the regression coefficient (R2) of 0.921 using CV. The sensor is highly specific, simple and takes only 30 min for detection of S. pyogenes infection. The sensor was further characterized by atomic force microscopy (AFM). Genosensor was found stable upto 6 months with only 10% loss in initial peak current in CV analysis on storage at 4°C. The speB gene based sensor can be used to prevent the damage of mitral and aortic heart valves by early diagnosis and treatment of the disease.
