Journal of Biotechnology & Biomaterials
黑料网
Our Group organises 3000+ Global Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ 黑料网 Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
黑料网 Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Citations : 3330
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Design and testing of duplex-PCR primers for detection of bacterial spot of tomato
2nd World Biotechnology Congress
Dagmar Stehlikova, Vladislav Curn and Pavel Beran
University of South Bohemia in Ceske Budejovice, Czech
Posters & Accepted Abstracts: J Biotechnol Biomater
DOI:
Abstract
This study presents development of duplex-PCR assay for specific detection of plant pathogenic bacteria of Xanthomonas genus causing bacterial spot of tomato and pepper. PCR primers for differentiation of X. euvesicatoria and X. vesicatoria were developed based on the DNA sequences of bacterial type strains. Primer pairs were designed and subsequently thoroughly tested and optimized for parallel detection of the bacteria. Specificity of the primers was tested on a large complex of bacterial strains pathogenic to tomato, pepper and related crops. Following the described protocol X. euvesicatoria and X. vesicatoria can be quickly and reliably identified in a single duplex-PCR assay.Biography
Dagmar Stehlíková is a Molecular Geneticist with specialization on detection phytopathogenic bacteria. She is most interested in quarantine bacteria Xanthomonas, Ralstonia, Burkholderia etc. Techniques that she is expert in molecular methods are loop-mediated isothermal amplification, reatime PCR and multipex PCR.
