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Abstract

Bacillus subtilis possesses excellent properties to be considered as a host for protein production. These properties include GRAS status and available large scale production. Also, this endotoxin-free bacterium confers significant advantages to producing recombinant protein products for animal and human uses. The development of these products requires efficient optional expression systems for this bacterium. Here, we will present a topic on the development of pHT expression vectors based on the promoter Pgrac family to produce recombinant proteins in B. subtilis, which can use in an IPTG-inducible or noninducible manners. First, we introduced a new synthetic promoter Pgrac derived from B. subtilis groESL promoter and E. coli lac operator, which can be induced by addition of IPTG. This promoter is 50 times stronger than Pspac, the most popular IPTGinducible promoter used for B.subtilis. Second, by changing the core promoter elements and the mRNA stabilizing elements to make an 84-promoter library, we showed that the second generation of Pgrac promoters could enhance production levels more than 30% of total cellular proteins in B. subtilis. Third, we generated potent expression vectors containing a second generation promoter of Pgrac family, which could be used to produce efficiently intracellular or extracellular recombinant proteins in B. subtilis. Finally, we will update recent development of novel non-inducible vectors using the IPTG-Pgrac promoter, which allowed production of recombinant proteins without the addition of IPTG. The use of IPTG-inducible and non-inducible expression vectors harboring Pgrac promoter will promise to bring enormous benefits to industrial applications.

Biography

Hoang Duc Nguyen has completed his PhD from University of Bayreuth in 2006 and Postdoctoral studies from Max Planck Institute for Molecular Physiology in 2011. He is appointed as an Associate Professor in 2015 and currently the Head of the Department of Microbiology and Director of Center for Bioscience and Biotechnology at VNUHCM-University of Science, Vietnam. He has published more than 20 papers in reputed journals.

Email: ndhoang@hcmus.edu.vn