黑料网

Abstract

Development of novel fl uorescent molecular probes with high performance is of much importance to detect proteins at high sensitivity. We designed and synthesized a fl uorescent molecule with both hydroxystyryl and cyanopyranyl moieties to detect the proteins via non-covalent bonding [1]. Th e most important and advantageous feature of this probe molecule is no fl uorescence emission in the absence of protein. Th e derivative with sulfonyl group has also been developed [2]. Th is probe is more soluble in an aqueous solution, and hence they can be applied to in-gel protein staining. In addition, we designed a more sensitive probe that is commercially available as Rapid FluoroStain KANTO (Kanto Chemical). In this study, we evaluated the performance of Rapid FluoroStain KANTO for in-gel protein staining. A fully automated two-dimensional electrophoresis (2DE) system developed by our group [3] was employed. Th is automated 2DE system can realize high-throughput proteomic analysis with high reproducibility and compact design. Sensitivity of protein staining was comparable with commercially available fl uorescent probes. It takes only 90 minutes to complete protein staining process, and this is much shorter than any other probes. In addition, we successfully designed a novel fl uorescent probe that is more sensitive than Rapid FluoroStain KANTO. We will report the details of the new probe at the conference.

Biography

Dr. Kenji Yokoyama is Deputy Director, Biotechnology Industrialization Center, National Institute of Advanced Industrial Science and Technology (AIST). He received his PhD at Tokyo Institute of Technology in 1991, and after postgraduate research at Research Center for Advanced Science and Technology, University of Tokyo, promoted to Associate Professor at School of Materials Science, Japan Advanced Institute of Science and Technology in 1994. He has moved to AIST since 2002. He has worked on electrochemical biosensors, peptide engineering and development of analytical tool for proteome