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Abstract

The recent progress in biotechnology has been made it possible to the combination of both protein and whole cell together to explore a wide range of applications. In this talk, the display of a series of enzymes such as xylose dehydrogenase, glucose dehydrogenase and organophosphorus hydrolase on the surface of Escherichia coli and glucose oxidase on yeast surface for the construction of whole-cell biocatalysts are demonstrated. The characterization of the fusion protein by proteinase accessibility, Western blot, and enzyme activity assays is performed. The optimal temperature and pH of the enzyme displayed cells are discussed. Finally, the sensitive and selective electrochemical biosensing of monosaccharides or organophosphates and assembly of biofuel cell on basis of the microbial surface displaying enzymes are highlighted. The enzyme-displayed bacterial cell can not only be used directly without further enzyme extraction and purification, but also it improves the stability of the enzyme. Therefore, microbial whole-cell would be engineered to develop novel approaches, which could be more efficient and cost-effective in comparison with traditional analytical techniques.

Biography

Aihua Liu is a Full Professor of Qingdao Institute of Bioenergy & Bioprocess, Chinese Academy of Sciences, where he leads the Laboratory of Biosensing. He received his PhD degree from Tohoku University, Japan (2004) with major in bioelectrochemistry and biosensing. Followed he worked in the National Institute of Advanced Industrial Science & Technology (AIST), Japan under the Japanese Society for the Promotion of Sciences (JSPS) fellowship (2004-2006). After that he moved to the US and conducted his postdoc research in Michigan State University (2006-2007), University of Oklahoma (2007-2009), and University of Texas at Arlington (2009-2010). His current research interests cover Microbial Surface Display, Biosensor, Biochips, and Biofuel Cells. He is the managing editor to Frontiers in Bioscience. He has published over 40 peer-reviewed papers in leading journals including AngewandteChemie International Edition, Advanced Materials, Analytical Chemistry, Biosensors & Bioelectronics and Journal of Molecular Biology.