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Direct Measurement of Lipase Inhibition by Orlistat Using a Dissolution Linked In Vitro Assay
Purpose: To develop a bio-assay that would be able to directly test gastrointestinal and/or dissolution samples to determine lipase activity and inhibition by Orlistat. Methods: Enzyme assays were performed with porcine pancreatic lipase and para-Nitrophenyl Palmitate (pNPP) in pH 8.0 reaction buffer at 37°C. Substrate hydrolysis was monitored... Read More»
Daniel R Lewis and Dongzhou J Liu
Research Article: Clin Pharmacol Biopharm 2012, 1:103
DOI:
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