Research Article
Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition
Storchilo OV*Odessa National Medical University, 2-Valyhovsky lane, Odessa, 65 082, Ukraine
- *Corresponding Author:
- Storchilo O.V
Odessa National Medical University
2-Valyhovsky lane, Odessa, 65 082, Ukraine
Tel: +38 (048)7255937
Fax: 063 308 50 29
E-mail: alena-61@mail.ru
Received date: June 4, 2015; Accepted date: July 11, 2015; Published date: July 20, 2015
Citation: Storchilo OV (2015) Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition. J Gastrointest Dig Syst 5:308. doi:10.4172/2161-069X.1000308
Copyright: © 2015 Storchilo OV. This is an open-access article distributed under the terms of the Creative Commons Attribution License; which permits unrestricted use; distribution; and reproduction in any medium; provided the original author and source are credited.
Abstract
Objectives and method: To determine the velocity of glycine absorption in the chronic experiments under physiological condition with no operation trauma, pain, narcosis and atrophy of the small intestine using and original method of surgical formation of the functioning fragment of the small intestine with ‘living fistulas’ in the presence of chime, all gastrointestinal secrets and natural innervations. Results: The glycine absorption velocity increases during one hour of perfusion. Absolute parameters of absorptive activity of the small intestine in the chronic experiments in vivo are higher than in the isolated loop of the rats’ small intestine. We observed no dissolution of perfusate with gastrointestinal fluids in the small intestine functioning part indicating that absorption of water in this fragment of small intestine prevails. Conclusions: Formation and perfusion of the functioning fragment of the rats’ small intestine is an adequate approach to the investigating the activity of the small intestine under physiological condition. It allows detecting the impact of the regulatory activity of chime (its exogenic and endogenic components).
