Research Article
MDR1 C3435T Polymorphism is a Susceptible Marker for the Late Onset Japanese UC
Sawasaki T, Nanjo S and Sugiyama T*Department of Gastroenterology, Graduate School of Medicine and Pharmaceutical Science, Sugitani 2630, Toyama City, Toyama 930-0194, Japan
- *Corresponding Author:
- Toshiro Sugiyama
MD, Ph.D., Department of Gastroenterology
Graduate School of Medicine and PharmaceuticalScience
Sugitani 2630, Toyama City, Toyama 930-0194, Japan
Tel: 81-76-434-7300
E-mail: tsugi@med.u-toyama.ac.jp
Received date: August 11, 2015 Accepted date: August 21, 2015 Published date: August 28, 2015
Citation: Sawasaki T, Nanjo S, Sugiyama T (2015) MDDR1 C3435T Polymorphism is a Susceptible Marker for the Late Onset Japanese UC. J Gastrointest Dig Syst 5:326. doi:10.4172/2161-069X.1000326
Copyright: © 2015 Sawasaki T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: The multi-drug resistance 1 (MDR1) gene is reported to correlate with the function and the expression of P-glycoprotein (P-gp), and the carriers of MDR1 C3435T polymorphism are speculated to have an increased susceptibility to ulcerative colitis (UC) in western countries. The aim of this study is to investigate whether MDR1 C3435T polymorphism is a susceptible marker for the onset of UC in Japanese patients. Patients and Methods:We obtained blood samples from 38 patients with UC and that from 76 healthy controls, and genomic DNA from leucocytes was extracted. MDR1 C3435T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism methods. The expression of P-gp was examined by immunohistochemistry in colonic tissues with UC. Results: Both the frequencies of TT genotype (p=0.023) and T allele (p=0.011) were significantly higher in the late onset UC groups than in the control group in Japanese UC patients. The protein of P-gp in colonic tissues showed lower expression in UC patients with TT genotype by immunohistological analysis. Conclusion: MDR1 C3435T polymorphisms are linked to the susceptibility of the late onset Japanese UC patients, probably via the lower exported activity of the related pathogenic environmental factors in colon.
