Prevalence and Diversity of Cag (PAI) in Helicobacter pylori: Study on Gastric Ulcer
Received Date: Apr 27, 2018 / Accepted Date: May 09, 2018 / Published Date: May 16, 2018
Abstract
Background: Frequency of H. Pylori in northern part of Iran within normal population is about 90% which is high, particular marker in order to diagnose patients with higher risk of peptic ulcer disease progression would be helpful for clinical practice. The purpose of this study was to evaluate the variable locus of CagA gene in virulence form.
Methods: For this aim 200 types of biopsy infected and positive urease test and 20 samples of healthy people biopsies in order for control were provided. After DNA purification, the variety of locus in CagA gene was confirmed by glmM, SSA and CagV primers.
Results: The results showed that 142 typical were positive for CagA. CagV primer used to variety fixed. The CagV gene exposed a special genetic variety in the 800 bp, 825 bp, 850 bp, 900 bp areas.
Conclusions: The result of study on this locus suggested that variation of CagV could be consider as a paradigm in intensity and distribution of the gastric ulcer disease and key marker to prevent progression or inhibition of the progress in gastrointestinal disease.
Keywords: CagA (PAI); CagV; H. pylori; PCR
Citation: Morshedzadeh F, Abbasinia H, Zaeifi D (2018) Prevalence and Diversity of Cag (PAI) in Helicobacter pylori: Study on Gastric Ulcer. J Gastrointest Dig Syst 8: 564. Doi: 10.4172/2161-069X.1000564
Copyright: © 2018 Morshedzadeh F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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