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Trapa Japonica Inhibits Adipocyte Differentiation and Adipogenesis through Ampk Signaling Pathway in 3t3-L1 Pre-Adipocytes

Cheol Park¹, Yong Hwang², Buyng Su Hwang², Su Young Shin², Pyo Yun Cho², Seung Young Lee², Kyung-Min Choi², Ki Won Lee³, Gi-Young Kim⁴, Yung Hyun Choi^5* and Jin-Woo Jeong^2*

¹Department of Molecular Biology, College of Natural Sciences, Dong-eui University, Busan 47340, Republic of Korea

²Nakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si, Gyeongsangbuk-do 37242, Republic of Korea

³Kwangdong Pharm Co., Ltd 1206, 271, Digital-ro, Guro-gu, Seoul, Republic of Korea

⁴Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea

⁵Department of Biochemistry, College of Korean Medicine, Dong-eui University, Busan 47227, Republic of Korea

Received Date: Oct 25, 2019 / Published Date: Aug 23, 2022

Citation: Park C, Hwang Y, Hwang BS, Shin SY, Cho PY, et al. (2022) Trapa Japonica Inhibits Adipocyte Differentiation and Adipogenesis through Ampk Signaling Pathway in 3t3-L1 Pre-Adipocytes. J Obes Weight Loss Ther 12: 513.DOI: 10.4172/2165-7904.1000513

Copyright: © 2022 Park C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Trapa japonica (water caltrop) is one of the main aquatic plant with various pharmacological functions; however,the effects associated with anti-obesity have not been fully identified. In the present study, we investigated the inhibition of adipocyte differentiation and adipogenesis by Hot-Water extracts of T. japonica (WETJ) in 3T3-L1 pre-adipocytes.Treatment with WETJ suppressed the terminal differentiation of 3T3-L1 pre-adipocytes in a dose-dependent manner,as confirmed by a decrease in the lipid droplet number and lipid content through Oil Red O staining. WETJ significantly reduced the accumulation of cellular triglyceride, which is associated with a significant inhibition of pro-adipogenic transcription factors, including PPARγ, C/EBPα and C/EBPβ. In addition, WETJ potentially downregulated the expression of adipocyte-specific genes, including aP2 and leptin. Furthermore, WETJ treatment effectively increased the phosphorylation of the AMPK and ACC; however, treatment with a potent inhibitor of AMPK, compound C, significantly restored the WETJ-induced inhibition of pro-adipogenic transcription factors. These results together indicate that WETJ has preeminent effects on the inhibition of adipogenesis through the AMPK signaling pathway, and further studies will be needed to identify the active compounds in T. japonica.