Research Article
Universal Primers for Exon-Priming Intron-Crossing (EPIC) PCR on Ribosomal Protein Genes in Marine Animals
Seinen Chow*, Takashi Yanagimoto and Yoji NakamuraNational Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, Japan
- *Corresponding Author:
- Seinen Chow
National Research Institute of Fisheries Science
2-12-4 Fukuura, Kanazawa
Yokohama, Kanagawa 236-8648, Japan
Tel: +81-45-788-7620
Fax: +81-45-788-5001
E-mail: chow@affrc.go.jp
Received date April 09, 2015; Accepted date May 23, 2015; Published date May 25, 2015
Citation: Chow S, Yanagimoto T, Nakamura Y (2015) Universal Primers for Exon-Priming Intron-Crossing (EPIC) PCR on Ribosomal Protein Genes in Marine Animals. J Marine Sci Res Dev 5:160. doi:10.4172/2155-9910.1000160
Copyright: © 2015 Chow S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Relatively conserved exon sequences among distant aquatic animal taxa (Copepoda, Echinodermata, and Teleostei) were determined for 12 ribosomal protein genes, and 19 primer pairs were designed for an exon-primed intron-crossing polymerase chain reaction strategy. The universal utility of these primers was evaluated in distant animal species (sea urchin, squid, crab, shark, and tuna). Fragment amplification was confirmed for at least three species for all primer pairs, and single fragment amplification was observed for at least one species in 16 primer pairs. Primer sets presented here may serve as an initial step for isolating single-copy nuclear DNA sequences in a wide variety of marine animals.
