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Abstract

Wheat leaf rust, caused by Puccinia triticina, endemic of South America, it is an important wheat disease. The Brazilian wheat cultivar Toropi has proven, durable adult plant resistance (APR) to leaf rust and prehaustorial resistance phenotype. The objective of this study was to identify, characterize and to understand the interaction between P. triticina and Toropi by quantitatively evaluating the temporal transcription genes related to infection and defense in wheat. RNA-Seq libraries from the Toropi here produced at 0, 6, 12, 24 hours after inoculation with P. triticina. Three replicates of each inoculated and control libraries were sequenced for each time point. A comparison to the Chinese Spring (CS42) transcriptome was performed aiming to identify putative genes unique to Toropi. A total of 58 million bp per library were produced: 10,181 contigs of which 9,156 were common to CS42 and 1,025 were unique to Toropi expressed only in the inoculated libraries. The expression profiles of 15 selected genes varied over time. Classical defense genes, including peroxidases, b-1,3-glucanases and an endochitinase were expressed (pre and post haustorial) over 72 hours infection time course, while induction of transcription of other infection related genes with a potential role in defense, although variable was maintained throughout. These genes had a role in plant lignification, oxidative stress, the regulation of energy supply, water and lipid transport and cell cycle regulation. These Toropi specific sequences could represent new resistance candidate genes to be used in wheat breeding and to guide further functional studies on APR to leaf rust.

Biography

E-mail: sandra.brammer@embrapa.br