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Aim: The aim of the present in vitro study was to evaluate the canal wall smear layer removal ability of SmearOFF, 7% maleic acid
(MA) and 18% ethylenediaminetetraacetic acid (EDTA).
Methods: Forty single-rooted human teeth were subjected to root canal instrumentation and were irrigated with 2.5% sodium
hypochlorite (NaOCl) between each instrument change. Samples were then divided into four groups based on final irrigation regimen:
[1] SmearOFF + 2.5% NaOCl, [2] 7% MA + 2.5% NaOCl, [3] 18% EDTA + 2.5% NaOCl, and [4] 0.9% saline (negative control). After
irrigation, the teeth were split longitudinally and examined by scanning electron microscopy (SEM). SEM images were then captured
and subjected to Image J software analysis to quantify the smear layer removal via measuring the amount of open dentinal tubules.
The open tubule percentage (OTP) was then calculated among the experimental irrigants.
Results: EDTA removed smear layer less efficiently when compared to SmearOFF and MA in all the thirds of the root canal system
(p<0.02). There was no significant difference between SmearOFF and MA in removal of smear layer from the coronal, middle and
apical thirds (p>0.06). In the negative control group (saline), all specimens were heavily smeared in the coronal, middle and apical
thirds of the root canal system. However, no statistical significance difference was observed when comparing EDTA and saline
(p>0.06).
Conclusion: SmearOFF and 7% MA had better canal wall smear layer removal capability when compared to 18% EDTA. There was
no difference between 18% EDTA and saline.