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Abstract

This study investigate the in vitro effects of newly synthesized hydrazones of N-pirolylcarboxylic acid on isolated rat brain synaptosomes. The main parameters, characterized the functional status of synaptosomes: synaptosomal viability and depletion of reduced glutathione (GSH), were measured. Administered alone, all the compounds (at concentration 50 μM) revealed statistically significant neurotoxic effects on the synaptosomes, compared to the control (non-treated synaptosomes). With lower toxic effects were compound DI5a and DI5g. These compounds (DI5a and DI5g) were examed for possible neuroprotective effects in a model of 6-hydroxydopamine (6-OHDA)- induced oxidative stress. The treatment of isolated rat brain synaptosomes with 6-OHDA is a reliable and commonly used in vitro model for the investigation of processes, which play role in the neurodegenerative disease, including Parkinson’s and Alzheimer’s disease. The mechanism of 6-OHDA neurotoxicity includes the formation of ROS and reactive metabolites, as a result of its metabolism in mitochondria of the neuronal cells. In conditions of 6-OH-dopamine-induced oxidative stress (at concentration 150 μM) on isolated rat synaposomes, DI5a and DI5g (at concentration 50 μM) revealed statistically significant neuroprotective effects by preservation the synaptosomal viability (measured by MTT-test), and GSH deletion, compared to the toxic agent.

Biography

Yoanna Koedzhikova is currently pursuing her Pharmacy at Faculty of Pharmacy of Medical University of Sofia. She has a long standing interest in Pharmacology and Toxicology and has worked over this scientific project to establish a scientifically important results over the influence of these new chemical compounds on the rat synaptozomes. She also has a deep knowledge in the sphere of preclinical and clinical studies of toxicity.

E-mail: yoanna.koedzhikova@gmail.com