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Abstract

Introduction: Head and neck squamous cell carcinoma (HNSCC) accounts for over 90% of all head and neck cancers. L-amino acid oxidases (LAAOs) are widely distributed in the venomous snake families Viperidae, Crotalidae and Elapidae. Several related proteins with LAAO activity have been described that exhibit antimicrobial, anti-neoplastic or apoptosis inducing activity. It is assumed that its general mechanism of toxicity is based on the generation of cytotoxic amounts of H2O2. Aim: The aim of this study was to determine the anticancer effect of LAAO on HEP-2 cell line after 24, 48 and 72 hours using flow cytometry and real time PCR. Materials & Methods: HEP-2 cell line is propagated and LAAO is added to the culture medium. The cells were assessed after 24, 48 and 72 hours. Results: The results of this study showed that LAAO caused apoptosis in HEP-2 cell line. The percentage of apoptosis was directly proportioned with time. The expression of p53 was increased by time while the expression of Bcl-2 decreased by time. Conclusion: These results indicate that LAAO have a powerful anticancer activity on HEP-2 cell line.

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