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Abstract

Neuroblastoma is often infiltrated by inflammatory cells. One possible role of these inflammatory cells is that they represent a cell-mediated immune response against cancer. CD8+ lymphocytes are a known crucial component of cellmediated immunity. This study was to explore the prognostic value of tumor-infiltrating CD8+ cytotoxic lymphocytes in Neuroblastoma. Tumor-infiltrating CD8+ lymphocytes were assessed by immune-histochemical staining of tumor tissue from 36 neuroblastoma from April 2008 to May 2015. The number of CD8+ T-cells was counted in tumor nest (intra-tumoral) and in the fibrovascular stroma of tumor (peritumoral) and their relationship with clinicopathologic outcome was determined. The total number of CD8+ cells was inversely correlated with tumor histology grade (P<0.001), vascular invasion (P<0.001), capsular invasion (P<0.002), calcification (P<0.005), necrosis of tumor (P<0.001), regional lymph nodes invasion (P<0.003), distant metastasis (P<0.003), stage (P<0.003) and was positive correlated with N-myc oncogene presentation (P<0.002) in neuroblastoma. However, there were no correlation between patient�s age, sex and size of tumor with infiltration of CD8+ cells (P<0.097, P<0.142 and P<0.722, respectively). In this analysis, total CD8 T-cell count was a dependent prognostic factor in children. Total number and stromal CD8 lymphocytes were associated with better patient survival (P<0.003 and P<0.05, respectively) in children. CD8 T lymphocytes have antitumor activity and influence the behavior of neuroblastoma and might be potentially being exploited in the treatment of neuroblastoma.

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