Journal of Gastrointestinal & Digestive System
黑料网
Our Group organises 3000+ Global Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ 黑料网 Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
黑料网 Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Citations : 2091
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- China National Knowledge Infrastructure (CNKI)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
Tobacco expression of novel envelope proteins-derived HBV antigens for cost-effective vaccine development
9th Euro Global Gastroenterology Conference
Mihaela-Olivia Dobrica, Lisa Paruch, Catalin Lazar, Hege Steen, Sissel Haugslien, Catalin Tucureanu, Iuliana Caras, Crina Stavaru, Norica Nichita and Jihong Liu Clarke
Institute of Biochemistry of the Romanian Academy, Romania Norwegian Institute of Bioeconomy Research, Norway The Cantacuzino National Institute for Research, Romania
Posters & Accepted Abstracts: J Gastrointest Dig Syst
DOI:
Abstract
Hepatitis B virus (HBV) infection represents a serious public health problem especially in developing countries. Despite the availability of a safe and efficient commercial vaccine, its use in mass immunization programs is hampered because of the high costs. Moreover, 10% of the vaccinated population develops a low immune response. In this context, we aim to design new HBV antigens in order to develop a more immunogenic vaccine in plants, as a low-cost alternative. Our strategy is focused on the antigenic properties of the large (L) envelope protein and on the ability of the small (S) surface antigen (HBsAg) to self-assemble into subviral particles (SVPs). HBsAg was used as a carrier for the 21-47 L-derived peptide by insertion in the antigenic loop (AGL) of HBsAg and by replacing a fragment from the AGL. The chimeric proteins along with the wild-type S protein were produced in green plants (Nicotiana benthamiana) and in HEK293T cells, as a reference system. Expression of the antigens was investigated in both production systems and properties like protein folding, dimerization and N-glycosylation were analyzed. The immunogenic properties of the newly produced antigens are currently investigated. To conclude, the new antigens represent promising candidates for the development of new vaccines against HBV.Biography
Mihaela-Olivia Dobrica was graduated in Biochemistry from University of Bucharest, Romania in 2012 and completed her Master’s degree in Neurobiology from University of Bucharest, Romania in 2014. She is currently pursuing PhD at the Institute of Biochemistry of the Romanian Academy, Department of Viral Glycoproteins.
