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Contamination of clinical settings to highly pathogenic Acanthamoeba in Iran
International Conference on Occupational Health & Safety Summit
Maryam Niyyati, Zohreh Lasjerdi, Ali Haghighi and Ehsan Nazemalhosseini Mojarad
ScientificTracks Abstracts: J Community Med Health Edu
DOI:
Abstract
Among many genera of free living Amoebae, Acanthamoeba spp arethe causative agent of diseases such as keratitis and encephalitis. Presence of pathogenic Acanthamoeba in clinical settings such as hospitals could be a health threat for patients and high-risk people. The aim of the present study was to screen for the presence of Acanthamoeba in dust and biofilm samples in clinical settings located in Tehran, using culturing and molecular approaches. A total of 112 dust and biofilm samples from wards of clinical settings were collected and examined for the presence of Acanthamoeba using culturing and molecular approaches. Briefly, samples were filtered and cultured on non nutrient agar covered with heat killed Escherichia coli. The presence of Acanthamoeba was confirmed by morphological criteria and genus-specific primer sets. Sequences were analyzed against all eukaryotic nucleotide sequences archived in the GenBank database. Pathogenicity of the strains were detected using thermotolerance and osmotolerance assays.Biography
Dr. Maryam Niyyati has completed her Ph.D at the age of 31 years from Tehran University of Medical Sciences and she has published more than 25 papers in reputed journals. She is now assistant professor of Shahid Beheshti University of Medical Sciences in Tehran, Iran.
